Sucrose Phosphorylase antibody (HRP)

Catalog No. ABIN5596915

Product Details anti-Sucrose Phosphorylase Antibody (HRP)

Target: Sucrose Phosphorylase

Reactivity: E. coli

Host: Goat

Clonality: Polyclonal

Conjugate: This Sucrose Phosphorylase antibody is conjugated to HRP

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Cross-Reactivity (Details): Cross reactivity against Sucrose Phosphorylase from other sources is unknown.

Purification: Sucrose Phosphorylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Sucrose Phosphorylase [E.coli].

Immunogen:

Immunogen: Sucrose Phosphorylase [E.coli]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Sucrose Phosphorylase has been assayed against 1.0 μg of Sucrose Phosphorylase [E.coli] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Sucrose Phosphorylase

Target: Sucrose Phosphorylase

Abstract: Sucrose Phosphorylase Products

Synonyms: sucrose phosphorylase antibody, Sucrose phosphorylase antibody, RB12343 antibody, Shewmr4_0064 antibody, Shewmr7_0062 antibody, Sfri_3990 antibody, Acid_2070 antibody, Shewana3_0066 antibody, BAD_RS00415 antibody, Pnap_0232 antibody, Shew185_0061 antibody, Blon_0128 antibody, Lm4b_00279 antibody, Apre_0241 antibody, EAMY_RS33260 antibody, Spirs_0911 antibody, SpiBuddy_2458 antibody, Lbuc_1855 antibody

Background:

Synonyms: Putative sucrose phosphorylase Sucrose glucosyltransferase

Background: Sucrose Phosphorylase is an important enzyme in the metabolism of sucrose and regulation of other metabolic intermediates. Sucrose phosphorylase is in the class of hexosyltransferases. It has been shown in multiple experiments that the enzyme catalyzes this conversion by a double displacement mechanism.

Gene Name: ycjM

Gene ID: 4492194

UniProt: P76041