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WECC antibody (HRP)

WECC Reactivity: Pseudomonas, Bacteria ELISA, IP, WB Host: Goat Polyclonal HRP
Catalog No. ABIN5596986
  • Target See all WECC products
    WECC (WecC Gene Product (WECC))
    Reactivity
    Pseudomonas, Bacteria
    Host
    • 3
    Goat
    Clonality
    • 3
    Polyclonal
    Conjugate
    • 2
    • 1
    This WECC antibody is conjugated to HRP
    Application
    ELISA, Immunoprecipitation (IP), Western Blotting (WB)
    Purification
    N-Acylmannosamine-1-Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, and purified and partially purified N-Acylmannosamine-1-Dehydrogenase derived from Pseudomonas.
    Immunogen

    Immunogen: N-Acyl Mannosamine-1-Dehydrogenase [Recombinant Sequence from Pseudomonas expressed in E.coli ]

    Immunogen Type: Native Protein

    Isotype
    IgG
  • Application Notes

    Application Note: Anti-N-Acylmannosamine-1-Dehydrogenase has been assayed against 1.0 μg of N-Acylmannosamine-1-Dehydrogenase IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 is suggested for this product.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    WECC (WecC Gene Product (WECC))
    Alternative Name
    N-Acylmannosamine-1-Dehydrogenase (WECC Products)
    Background

    Synonyms: N-acylmannosamine 1-dehydrogenase NAM-DH EC=1.1.1.233

    Background: N-Acylmannosamine-1-Dehydrogenase acts on acetyl-D-mannosamine and glycolyl-D-mannosamine. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.

    Gene Name: NAM-DH

    Gene ID
    7614553
    UniProt
    P22441
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