Clusterin antibody
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- Target See all Clusterin (CLU) Antibodies
- Clusterin (CLU)
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Clusterin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)
- Cross-Reactivity (Details)
- Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein J and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, C-III and E apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
- Purification
- This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.
- Immunogen
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Immunogen: apoLipoprotein Type J was isolated from human plasma by density gradient centrifugation followed by HPLC purification.
Immunogen Type: Native Protein
- Isotype
- IgG
- Top Product
- Discover our top product CLU Primary Antibody
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- Application Notes
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Immunohistochemistry Dilution: 1:50 - 1:200
Application Note: Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.
Western Blot Dilution: 1:5,000 - 1:10,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:10,000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Stabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
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Protection of neurons from apoptosis by apolipoprotein E-containing lipoproteins does not require lipoprotein uptake and involves activation of phospholipase Cgamma1 and inhibition of calcineurin." in: The Journal of biological chemistry, Vol. 284, Issue 43, pp. 29605-13, (2009) (PubMed).
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Protection of neurons from apoptosis by apolipoprotein E-containing lipoproteins does not require lipoprotein uptake and involves activation of phospholipase Cgamma1 and inhibition of calcineurin." in: The Journal of biological chemistry, Vol. 284, Issue 43, pp. 29605-13, (2009) (PubMed).
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- Target
- Clusterin (CLU)
- Alternative Name
- Apolipoprotein J (CLU Products)
- Synonyms
- LOC100136120 antibody, clu antibody, MGC53055 antibody, zgc:73352 antibody, xclu antibody, MGC89163 antibody, CLU antibody, APO-J antibody, APOJ antibody, CLI antibody, CLU1 antibody, CLU2 antibody, KUB1 antibody, NA1/NA2 antibody, SGP-2 antibody, SGP2 antibody, SP-40 antibody, TRPM-2 antibody, TRPM2 antibody, DAG antibody, RATTRPM2B antibody, SP40 antibody, TRPM2B antibody, Trpm2 antibody, Trpmb antibody, AI893575 antibody, ApoJ antibody, Cli antibody, D14Ucla3 antibody, Sgp-2 antibody, Sgp2 antibody, Sugp-2 antibody, GP80 antibody, gpIII antibody, 40 antibody, clusterin-1 antibody, clusterin L homeolog antibody, clusterin antibody, Clusterin antibody, LOC100136120 antibody, clu.L antibody, clu antibody, CLU antibody, clus antibody, Clu antibody, LOC101113728 antibody
- Background
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Synonyms: Clusterin, Complement-associated protein SP-40,40, Complement cytolysis inhibitor, CLI, NA1/NA2, Apolipoprotein J, Apo-J, Testosterone-repressed prostate message 2, TRPM-2, Ku70-binding protein 1, Aging-associated gene 4 protein
Background: This antibody is suitable for cardiovascular research. Cleaved into the following 2 chains: 1- Recommended name: Clusterin beta chain Alternative name(s): ApoJalpha Complement cytolysis inhibitor a chain 2- Recommended name: Clusterin alpha chain Alternative name(s): ApoJbeta Complement cytolysis inhibitor b chain
Gene Name: CLU, APOJ, CLI, KUB1
- Gene ID
- 1191
- UniProt
- P10909
- Pathways
- Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
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