Goat anti-Ferret IgG (Heavy Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed
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- Target See all IgG products
- IgG
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Binding Specificity
- Heavy Chain
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Reactivity
- Goat, Ferret
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Alkaline Phosphatase (AP)
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Characteristics
- Ferret IgG (gamma chain) antibody detects the gamma chain of ferret IgG molecule. Product showed no reactivity with IgG from other similar species. Anti-Ferret IgG (gamma chain) antibody is ideal for investigators involved in serum component protein research.
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
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Immunogen: Ferret IgG gamma heavy chain
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Ferret IgG (gamma chain) antibody is suitable for ELISA, Western Blot and Immunohistochemistry. Antibody should be optimized by end user for reactive conditions.
ELISA Dilution: 1:2,000 - 1:10,000
Western Blot Dilution: 1:500 - 1:2,500
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
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Synonyms: goat anti-Ferret IgG Antibody alkaline phosphatase Conjugation, goat anti-Ferret IgG alk phos Conjugated Antibody
Background: Anti-Ferret IgG Alkaline Phosphatase antibody generated in goat detects ferret IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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