PCNA antibody (AA 111-122) (Biotin)

Catalog No. ABIN5608780

This anti-PCNA antibody is a Goat Polyclonal antibody detecting PCNA in WB and ELISA. Suitable for Human.

Quick Overview for PCNA antibody (AA 111-122) (Biotin) (ABIN5608780)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PCNA antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PCNA Antibody (Biotin)

Binding Specificity: AA 111-122

Purpose: PCNA (aa111-122), Biotinylated

Sequence: EAPNQEKVSD YE.

Specificity: Reported variants represent identical protein: NP_872590.1, NP_002583.1

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 30 kDa band observed in lysates of cell line Jurkat (calculated MW of 28.8 kDa according to Human NP_002583.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Gilljam et al, , J C

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background: PCNA, proliferating cell nuclear antigen, MGC8367, DNA polymerase delta auxiliary protein, OTTHUMP00000030189, OTTHUMP00000030190, cyclin

Gene ID: 5111, 18538, 25737

NCBI Accession: NP_002583

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy