BRAF antibody (V600E)

Catalog No. ABIN5647056

This Rabbit Polyclonal antibody specifically detects BRAF in WB. It exhibits reactivity toward Human.

Quick Overview for BRAF antibody (V600E) (ABIN5647056)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRAF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BRAF Antibody

Binding Specificity: AA 576-606, V600E

Cross-Reactivity (Details): Expected species reactivity: Mouse,Rat,Bovine,Chicken

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 576-606 from human BRAF was used as the immunogen for the BRAF V600E antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the BRAF V600E antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. WB: 1:1000-1:2000

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the BRAF V600E antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: BRAF V600E

Background: Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

UniProt: P15056

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy