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Recombinant BRAF antibody (N-Term)
The Rabbit Monoclonal anti-BRAF antibody has been validated for WB and IHC. It is suitable to detect BRAF in samples from Human.
Quick Overview for Recombinant BRAF antibody (N-Term) (ABIN5647699)
Target
See all BRAF Antibodies
BRAF
(B-Raf proto-oncogene, serine/threonine kinase (BRAF))
Antibody Type
Recombinant Antibody
Reactivity
All reactivities for BRAF antibodies
Human
Host
All hosts for BRAF antibodies
Rabbit
Clonality
All clonalities for BRAF antibodies
Monoclonal
Conjugate
All conjugates for BRAF antibodies
This BRAF antibody is un-conjugated
Application
All applications for BRAF antibodies
Western Blotting (WB), Immunohistochemistry (IHC)
Clone
RM308
Product Details anti-BRAF Antibody
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Binding Specificity
All epitopes for BRAF antibodies
N-Term
Cross-Reactivity (Details)
Expected species reactivity: Mouse
Characteristics
This antibody reacts to human serine/threonine-protein kinase B-raf (BRAF). It may also react to mouse B-raf, as predicted by immunogen homology.
Purification
Purified
Immunogen
A peptide corresponding to the residues near N-terminus of human B-raf was used as the immunogen for the recombinant BRAF antibody.
Isotype
IgG
Alternatives
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Application Details
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Application Notes
The stated application concentrations are suggested starting points. Titration of the recombinant BRAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunohistochemistry (FFPE): 1:200-1:500,Western Blot: 1:100-1:200
Restrictions
For Research Use only
Handling
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Buffer
Antibody in PBS with 50 % Glycerol, 1 % BSA and 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Store the recombinant BRAF antibody at -20°C.
Target Details for BRAF
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Target
BRAF
(B-Raf proto-oncogene, serine/threonine kinase (BRAF))
Alternative Name
BRAF
Background
This antibody reacts to human serine/threonine-protein kinase B-raf (BRAF). It may also react to mouse B-raf, as predicted by immunogen homology.
UniProt
P15056
Pathways
MAPK Signaling , RTK Signaling , Neurotrophin Signaling Pathway , Ribonucleoprotein Complex Subunit Organization , Hepatitis C , Autophagy
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