Crotonaldehyde (CRA) antibody (HRP)

Catalog No. ABIN5650478

Product Details

Target: Crotonaldehyde (CRA)

Host: Mouse

Clonality: Monoclonal

Conjugate: HRP

Application: Western Blotting (WB), ELISA

Specificity: Specific for Crotonaldehyde modified proteins. Does not cross-react with Acrolein, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.

Purification: Protein G Purified

Immunogen: Crotonaldehyde modified Keyhole Limpet Hemocyanin (KLH)

Clone: 2A8-1

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• ELISA (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5650478 was sufficient for detection of Crotonaldehyde-modified proteins in 1 μg of Crotonaldehyde-BSA conjugate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details

Target: Crotonaldehyde (CRA)

Alternative Name: Crotonaldehyde

Target Type: Chemical

Background: Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. Crotonaldehyde (CRA) is a carcinogenic, conjugated aldehyde produced endogenously via lipid peroxidation. It covalently modifies biomolecules, forming adducts with DNA and nucleophilic amino acid residues of proteins such as lysine (1).