Crotonaldehyde (CRA) antibody (PerCP)
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- Target
- Crotonaldehyde (CRA)
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- PerCP
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Application
- Western Blotting (WB), ELISA
- Specificity
- Specific for Crotonaldehyde modified proteins. Does not cross-react with Acrolein, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
- Purification
- Protein G Purified
- Immunogen
- Crotonaldehyde modified Keyhole Limpet Hemocyanin (KLH)
- Clone
- 2A8-1
- Isotype
- IgG1
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- Application Notes
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- WB (1:1000)
- ELISA (1:1000)
- optimal dilutions for assays should be determined by the user.
- Comment
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A 1:1000 dilution of ABIN5650482 was sufficient for detection of Crotonaldehyde-modified proteins in 1 μg of Crotonaldehyde-BSA conjugate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Target
- Crotonaldehyde (CRA)
- Alternative Name
- Crotonaldehyde
- Target Type
- Chemical
- Background
- Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. Crotonaldehyde (CRA) is a carcinogenic, conjugated aldehyde produced endogenously via lipid peroxidation. It covalently modifies biomolecules, forming adducts with DNA and nucleophilic amino acid residues of proteins such as lysine (1).
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