KDEL antibody (N-Term) (HRP)

Catalog No. ABIN5650528

Product Details anti-KDEL Antibody (HRP)

Target: KDEL (Lys Asp Glu Leu)

Binding Specificity: N-Term

Reactivity: Rat, Mouse, Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KDEL antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Detects KDEL tagged proteins.

Purification: Protein G Purified

Immunogen: KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)

Clone: 2C1

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:25)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5650528 was sufficient for detection of KDEL in 10 μg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium Azide

Storage: -20 °C

Target Details for KDEL

Target: KDEL (Lys Asp Glu Leu)

Alternative Name: KDEL (Lys Asp Glu Leu Products)

Background: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.