4-Hydroxy-2-Hexenal (4-HHE) antibody (HRP)
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- Target
- 4-Hydroxy-2-Hexenal (4-HHE)
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- HRP
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Application
- Western Blotting (WB), ELISA
- Specificity
- Specific for 4-HHE. Slight cross-reactivity with 4-Hydroxynonenal. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, MDA, or Methylglyoxal modified proteins.
- Purification
- Protein G Purified
- Immunogen
- Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH)
- Clone
- 5C11-1
- Isotype
- IgG1
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- Application Notes
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- WB (1:1000)
- ELISA (1:1000)
- optimal dilutions for assays should be determined by the user.
- Comment
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A 1:1000 dilution of ABIN5650568 was sufficient for detection of 4-Hydroxy-2-hexenal in 1 μg of 4-Hydroxy-2-hexenal-BSA conjugate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Target
- 4-Hydroxy-2-Hexenal (4-HHE)
- Alternative Name
- 4-Hydroxy-2-hexenal
- Target Type
- Chemical
- Background
- Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. 4-hydroxy-2-hexenal (4-HHE) is a major alpha,beta-unsaturated aldehyde product of n-3 PUFA oxidation resulting from lipid peroxidation. High levels of HHE are found in disease states (1). 4-HHE reacts with histidine residues to form Michael-addition type adducts.
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