c-FOS antibody (AA 280-380) (PE)
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- Target See all c-FOS (c-Fos) Antibodies
- c-FOS (c-Fos) (c-Fos)
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Binding Specificity
- AA 280-380
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This c-FOS antibody is conjugated to PE
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Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Specificity
- Expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, they undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the protein becomes nuclear.,Detects 62 kDa band. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.
- Cross-Reactivity
- Human, Rat
- Purification
- Protein G Purified
- Immunogen
- Synthetic peptide from the N-terminal of Human c-Fos (aa. 280-380)
- Clone
- 4A3
- Isotype
- IgG2
- Top Product
- Discover our top product c-Fos Primary Antibody
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anti-c-Fos (c-Fos) antibody
c-Fos Reactivity: Human WB, ELISA, IHC, FACS Host: Mouse Monoclonal 2G2 unconjugated
anti-c-Fos (c-Fos) (AA 283-295) antibodyVerified c-Fos Reactivity: Human ELISA, IHC, IF Host: Goat Polyclonal unconjugated
anti-c-Fos (c-Fos) antibodyc-Fos Reactivity: Human WB, ELISA, IHC, FACS, IF Host: Rabbit Monoclonal 14C10 unconjugated Recombinant Antibody
anti-c-Fos (c-Fos) antibodyc-Fos Reactivity: Human, Mouse, Rat IHC Host: Rabbit Polyclonal unconjugated
anti-c-Fos (c-Fos) (N-Term) antibodyc-Fos Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC, IP Host: Rabbit Polyclonal unconjugated
anti-c-Fos (c-Fos) antibodyc-Fos Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Mouse Monoclonal 4B4F11 unconjugated
anti-c-Fos (c-Fos) (AA 211-380) antibodyc-Fos Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated
anti-c-Fos (c-Fos) (AA 1-380) antibodyc-Fos Reactivity: Human WB, ELISA, PLA Host: Mouse Monoclonal 2D11 unconjugated
anti-c-Fos (c-Fos) antibodyc-Fos Reactivity: Human, Mouse, Rat WB, IF Host: Rabbit Polyclonal unconjugated
anti-c-Fos (c-Fos) (N-Term) antibodyc-Fos Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
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- Application Notes
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- WB (1:1000)
- optimal dilutions for assays should be determined by the user.
- Comment
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A 1:1000 dilution of ABIN5650659 was sufficient for detection of c-fos in 10 μg of serum starved HeLa cells treated with PMA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Target
- c-FOS (c-Fos) (c-Fos)
- Alternative Name
- c-fos (c-Fos Products)
- Synonyms
- AP-1 antibody, C-FOS antibody, p55 antibody, c-fos antibody, D12Rfj1 antibody, cFos antibody, cFOS antibody, fos antibody, Fos proto-oncogene, AP-1 transcription factor subunit antibody, FBJ osteosarcoma oncogene antibody, proto-oncogene c-Fos-like antibody, FOS antibody, Fos antibody, LOC101078177 antibody
- Gene ID
- 2353
- NCBI Accession
- NP_005243
- UniProt
- P01100
- Pathways
- S100 Proteins
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