MED15 antibody (AA 1-88)

Catalog No. ABIN565638

Cited in 2+ publications. The Mouse Monoclonal anti-MED15 antibody (Clone 4A4) (ABIN565638) specifically detects MED15 in WB, ELISA and IF. The antibody is reactive with Human samples.

Quick Overview for MED15 antibody (AA 1-88) (ABIN565638)

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MED15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: 4A4

Product Details anti-MED15 Antibody

Binding Specificity: AA 1-88

Purpose: Mouse monoclonal antibody raised against a partial recombinant PCQAP.

Sequence: MDVSGQETDW RSTAFRQKLV SQIEDAMRKA GVAHSKSSKD MESHVFLKAK TRDEYLSLVA RLIIHFRDIH NKKSQASVSD PMNALQSL

Cross-Reactivity: Human, Mouse

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: PCQAP (NP_056973, 1 a.a. ~ 88 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-MED15 antibody (ABIN565638)

Ding, Tomomori-Sato, Sato, Conaway, Conaway, Boyer: "MED19 and MED26 are synergistic functional targets of the RE1 silencing transcription factor in epigenetic silencing of neuronal gene expression." in: The Journal of biological chemistry, Vol. 284, Issue 5, pp. 2648-56, (2009) (PubMed).

Varelas, Sakuma, Samavarchi-Tehrani, Peerani, Rao, Dembowy, Yaffe, Zandstra, Wrana: "TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal." in: Nature cell biology, Vol. 10, Issue 7, pp. 837-48, (2008) (PubMed).

Target Details for MED15

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Alternative Name: MED15

Background: Full Gene Name: mediator complex subunit 15
Synonyms: ARC105,CAG7A,CTG7A,DKFZp686A2214,DKFZp762B1216,FLJ42282,FLJ42935,PCQAP,TIG-1,TIG1,TNRC7

Gene ID: 51586

NCBI Accession: NM_015889

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha