Recombinant His Tag antibody (N-Term) (Biotin)

Catalog No. ABIN5671300

The Biotin-conjugated Rabbit Monoclonal anti-His Tag antibody (Clone RM146) (ABIN5671300) specifically detects His Tag in WB, IP, IHC, FACS and ICC.

Quick Overview for Recombinant His Tag antibody (N-Term) (Biotin) (ABIN5671300)

Target: His Tag

Antibody Type: Recombinant Antibody

Host: Rabbit

Clonality: Monoclonal

Conjugate: This His Tag antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: RM146

Product Details anti-His Tag Antibody (Biotin)

Binding Specificity: N-Term

Purpose: Biotinylated Rabbit monoclonal to His-Tag (6xHis-Tag or 10xHis-Tag)

Specificity: This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.

Purification: Protein A affinity purified from an animal origin-free culture supernatant

Immunogen: Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.

Isotype: IgG

Application Details

Application Notes: Western Blot: 0.1 μg/mL - 0.5 μg/mL, Immunocytochemistry 0.5 μg/mL - 2 μg/mL, Flow Cytometry: 0.5 μg/mL - 2 μg/mL, Immunohistochemistry, 0.1 μg/mL-1 μg/mL, ELISA: 0.01 μg/mL-0.5 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 50 % Glycerol/PBS with 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20.0°C

Target Details for His Tag

Target: His Tag

Alternative Name: His-Tag

Target Type: Tag