Collagen Type I antibody

Catalog No. ABIN5680212

This Rabbit Polyclonal antibody specifically detects Collagen Type I in WB, ELISA, IF, IHC (p) and RIA. It exhibits reactivity toward Cow.

Quick Overview for Collagen Type I antibody (ABIN5680212)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Product Details anti-Collagen Type I Antibody

Specificity: Bovine collagen type I 100 % , bovine collagen II, IX, XI <0.1 %, sheep collagen type I: 50 % (IHC estimated), bovine fibronectin <0.1 %, human collagen type I <2 %, chicken collagen type I <0.1 % (estimates except sheep collagen I from solid phase RIA at dilution 1:500) Cross-reacting with sheep

Cross-Reactivity (Details): Cattle, cross-reacting with sheep, swine and human (IHC)

Characteristics: Type I Collagen usually exists as a heterotrimer formed by alpha 1(I) and alpha 2(I) chains and is found in bone, cornea, skin and tendon. In foetal tissues also homotrimers of alpha-1(I) are found, but they are not constituents of normal adult tissues. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Bovine collagen type I 100 % , bovine collagen II, IX, XI <0.1 %, sheep collagen type I: 50 % (IHC estimated), bovine fibronectin <0.1 %, human collagen type I <2 %, chicken collagen type I <0.1 % (estimates except sheep collagen I from solid phase RIA at dilution 1:500).

Purification: Affinity purified

Immunogen: Purified collagen type I from bovine skin

Isotype: IgG

Application Details

Application Notes: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Bovine skin or liver,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:1000, ELISA ? 1:400 (OD ? 500)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 1 mg/mL

Buffer: phosphate buffered solution, no BSA and preservative added!

Preservative: Without preservative

Storage: -20 °C

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen Type I