COL2 antibody
-
- Target See all COL2 Antibodies
- COL2 (Collagen, Type II (COL2))
-
Reactivity
- Cow
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This COL2 antibody is un-conjugated
-
Application
- ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA)
- Specificity
- Bovine collagen type II 100 % , bovine collagen XI <3.0 %, bovine collagen I and IX <0.1 % (RIA at 1:200 dilution) (cross-reacting with horse and pig)
- Cross-Reactivity
- Horse (Equine), Pig (Porcine)
- Cross-Reactivity (Details)
- Cattle, (cross-reacting with horse and pig)
- Characteristics
- Type II collagen is an alpha1(II)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in cartilage and various tumours. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Bovine collagen type II 100 % , bovine collagen XI <3.0 %, bovine collagen I and IX <0.1 % (RIA at 1:200 dilution).
- Purification
- Affinity purified
- Immunogen
- Purified collagen type II from bovine cartilage
- Isotype
- IgG
- Top Product
- Discover our top product COL2 Primary Antibody
-
-
- Application Notes
- After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Bovine cartilage,IHC(P), IFA, ELISA, RIA,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA 1:40, IHC(P) 1:100 - 1:500, ELISA ? 1:200 (OD ? 500)
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Concentration
- 1 mg/mL
- Buffer
- phosphate buffered solution, no BSA and preservative added!
- Preservative
- Without preservative
- Storage
- -20 °C
-
- Target
- COL2 (Collagen, Type II (COL2))
- Alternative Name
- Collagen Type II (COL2 Products)
- Synonyms
- PT antibody, RPRGL2 antibody, THPH1 antibody, Cf-2 antibody, Cf2 antibody, FII antibody, thrombin antibody, prothrombin antibody, CAP18 antibody, Cnlp antibody, Cramp antibody, FALL39 antibody, MCLP antibody, coagulation factor II, thrombin antibody, coagulation factor II antibody, cathelicidin antimicrobial peptide antibody, Cuticle collagen 2 antibody, collagen type II alpha 1 chain antibody, F2 antibody, Camp antibody, col-2 antibody, COL2A1 antibody
-