This antibody is expected to recognize all reported isoforms (NP_001123476.1, NP_001093.1, NP_001123477.1).
Purification
ACTN1 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ACTN1 Antibody was raised against synthetic peptide TPQEINGKWDHVRQ found at an internal region of ACTN1.
ACTN1
Reactivity: Mouse, Rat
WB, IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 100 kDa band observed in Human, Mouse and Rat Spleen lysates (calculated MW of 103 kDa according to NP_001093.1). Recommended concentration: 0.3-1μ,g/mL. An additional band of unknown identity was also consistently observed at 75 kDa. Th
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
ACTN1 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.