This alpha 2 Macroglobulin antibody is un-conjugated
Application
Western Blotting (WB), ELISA
Purification
alpha-2-macroglobulin Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
alpha-2-macroglobulin Antibody was raised against synthetic peptide TSLTVRVNYKDRSP found at an internal region of alpha-2-macroglobulin.
A2M
Reactivity: Human
WB
Host: Mouse
Polyclonal
unconjugated
Application Notes
ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 200 kDa band observed in Human Albumin-depleted Serum lysates (calculated MW of 163 kDa according to NP_000005.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.5-2μ,g/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
alpha-2-macroglobulin is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.