RANKL antibody (Biotin)

Catalog No. ABIN5687612

Product Details anti-RANKL Antibody (Biotin)

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RANKL antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Purification: Anti-hsRANKL specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hsRANKL (Human sRANK-Ligand).

Application Details

Application Notes: ELISA:

To detect hsRANKL by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hsRANKL.

Sandwich:

To detect hsRANKL by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human sRANKL (XP-5274) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsRANKL.

Western Blot:
To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: sRANK Ligand antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for RANKL

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Alternative Name: sRANK Ligand (TNFSF11 Products)

Synonyms: CD254 antibody, ODF antibody, OPGL antibody, OPTB2 antibody, RANKL antibody, TRANCE antibody, hRANKL2 antibody, sOdf antibody, TNFSF11 antibody, Ly109l antibody, OPG antibody, Trance antibody, cd254 antibody, odf antibody, opgl antibody, optb2 antibody, rankl antibody, sodf antibody, tnfsf11 antibody, trance antibody, TNF superfamily member 11 antibody, tumor necrosis factor (ligand) superfamily, member 11 antibody, TNF receptor superfamily member 11b antibody, TNF superfamily member 11 L homeolog antibody, TNFSF11 antibody, Tnfsf11 antibody, TNFRSF11B antibody, tnfsf11.L antibody

Gene ID: 8600

UniProt: O14788

Pathways: NF-kappaB Signaling