IGF1 antibody (Biotin)

Catalog No. ABIN5687628

This Goat Polyclonal antibody specifically detects IGF1 in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for IGF1 antibody (Biotin) (ABIN5687628)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IGF1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-IGF1 Antibody (Biotin)

Purification: Anti-mIGF-I specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mIGF-I (murine IGF-I).

Application Details

Application Notes: ELISA:

To detect mIGF-I by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mIGF-I.

Sandwich

To detect mIGF-I by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine IGF-I (XP-5159) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIGF-I.

Western Blot:

To detect mIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIGF-I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: IGF-I antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: IGF-I

Gene ID: 16000

UniProt: P05017

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation