apolipoprotein D Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
apolipoprotein D Antibody was raised against synthetic peptide EIEKIPTTFENGRC found at an internal region of apolipoprotein D.
APOD
Reactivity: Human, Mouse, Rat
WB, ELISA, ICC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 25 kDa band observed in Human Liver and Kidney lysates (calculated MW of 21.3 kDa according to NP_001638.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1μ,g/mL. Immunohistochemistry: In paraffin embedded Human Kidney shows staining of the brush border of PCT. Recommended concentration, 5-10μ,g/mL. Enzyme immunoassay:This antibody was reported to work in sandwich-type ELISA both as a capture and detection antibody.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
apolipoprotein D is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.