This antibody is expected to recognize all reported isoforms (NP_004037.1, NP_001244263.1, NP_001001935.1). Reported variants represent identical protein: NP_001244264.1, NP_001001935.1. Reported variants represent identical protein: NP_004037.1, NP_00100
Purification
ATP5A1 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ATP5A1 Antibody was raised against synthetic peptide RVHGLRNVQAEE found at an internal region (near the N Terminus) of ATP5A1.
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 55 kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 54.5 kDa according to NP_001001935.1). Recommended concentration: 0.01-0.03μ,g/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
ATP5A1 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.