This antibody is expected to recognize both reported isoforms (NP_001190.1, NP_006120.1).
Purification
BMP1 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
BMP1 Antibody was raised against synthetic peptide DTIVPKYEVNGVK found at an internal region of BMP1.
BMP1
Reactivity: Human
WB, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: antibody detection limit dilution 1:128000. Western Blot: 1μ,g/mL staining of Human Heart and Kidney lysates, 2μ,g/mL staining of Rat Heart lysate, and 2μ,g/mL staining of NIH3T3 cell lysate (35μ,g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
BMP1 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.