This antibody is expected to recognize isoform 1 (NP_001738.2) only. Reported variants represent identical protein: NP_001243068.1, NP_001738.2.
Purification
CAPG Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
CAPG Antibody was raised against synthetic peptide KAQVEIVTDGEEP found at an internal region of CAPG.
Verified
CAPG
Reactivity: Human
WB, ELISA
Host: Goat
Polyclonal
unconjugated
Application Notes
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 38 kDa band observed in lysates of cell line U937 and of Human, Mouse and Rat Spleen (calculated MW of 38.5 kDa according to NP_001738.2). In Peripheral Blood Lymphocytes, the observed band runs as ~40 kDa. Recommended concentration: 0.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
CAPG is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.
Target
CAPG
(Capping Protein (Actin Filament), Gelsolin-Like (CAPG))