CD3EAP Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
CD3EAP Antibody was raised against synthetic peptide PEDKTVKQEQINTEP found at an internal region of CD3EAP.
CD3EAP
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
ELISA: antibody detection limit dilution 1:4000. Western Blot: Preliminary experiments in nuclear lysates of cell lines HeLa, Jurkat and A431 gave no specific signal but low background (at antibody concentration up to 1μ,g/mL).
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
CD3EAP is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.
Target
CD3EAP
(CD3e Molecule, epsilon Associated Protein (CD3EAP))