COL4A4 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
COL4A4 Antibody was raised against synthetic peptide SEEAIRPYVSR found at an internal region (near the C Terminus) of COL4A4.
Col4A4
Reactivity: Human
ELISA, WB, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments in lysates of several human tissues and cell lines gave no specific signal but low background (at antibody concentration up to 1μ,g/mL).
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
COL4A4 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.
Target
Col4a4 (Col4A4)
(Collagen, Type IV, alpha 4 (Col4A4))