This antibody is expected to recognize both reported isoforms (NP_001164645.1, NP_001328.1). Reported variants represent identical protein: NP_001164643.1, NP_001328.1, NP_001164642.1.
Purification
CX3CR1 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
CX3CR1 Antibody was raised against synthetic peptide DQFPESVTENFEYD found at an internal region (near the N Terminus) of CX3CR1.
Verified
CX3CR1
Reactivity: Human
WB, ELISA
Host: Goat
Polyclonal
unconjugated
Application Notes
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 39 kDa band observed in lysates of cell line Kelly and 37 kDa in lysates of cell line U251-MG (calculated MW of 40.4 kDa according to NP_001328.1). Recommended concentration: 1-3μ,g/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
CX3CR1 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.