Western Blotting (WB), ELISA, Enzyme Immunoassay (EIA)
Purification
cytokeratin 20 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
cytokeratin 20 Antibody was raised against synthetic peptide NSRHTVNYGSDLT found at an internal region of cytokeratin 20.
KRT20
Reactivity: Human
IHC, StM
Host: Mouse
Monoclonal
KRT20-3145
unconjugated
Application Notes
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 49 kDa band observed in Human Colon and Ileum lysates (calculated MW of 48.5 kDa according to NP_061883.1). Recommended concentration: 0.01-0.03μ,g/mL. Enzyme immunoassay:This antibody was reported to work in sandwich-type ELISA as a capture antibody.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
cytokeratin 20 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.