This antibody is expected to recognize isoform 1 (NP_001135939.1) only.
Purification
HES4 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
HES4 Antibody was raised against synthetic peptide RSAAEHRKVGSRP found at an internal region of HES4.
HES4
Reactivity: Human
WB, ELISA, IC
Host: Sheep
Polyclonal
unconjugated
Application Notes
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 24 kDa band observed in nuclear lysates of cell line HepG2 (calculated MW of 26.1 kDa according to NP_001135939.1). Recommended concentration: 1-3μ,g/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
HES4 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.