This antibody is expected to recognize all reported isoforms (NP_001095886.1, NP_000884.1, NP_001159923.1).
Purification
kininogen 1 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
kininogen 1 Antibody was raised against synthetic peptide RITEATKTVGSDT found at an internal region (near the N Terminus) of kininogen 1.
KNG1
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 80+60+30 kDa bands observed in Human Kidney lysates (calculated MW of 72.0 kDa according to NP_001095886.1). The observed MW bands are explained by glycosylation and the splitting into heavy and light chains from the precursor protein.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
kininogen 1 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.