This antibody is expected to recognize isoform 1 (NP_877421.2) only.
Purification
NBL1 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
NBL1 Antibody was raised against synthetic peptide KLGKTHGHRALE found at an internal region of NBL1.
NBL1
Reactivity: Mouse
ELISA, IF (cc), IF (p), IHC (fro), IHC (p)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 25 kDa band observed in lysates of cell line Kelly (calculated MW of 23.2 kDa according to NP_877421.2). Recommended concentration: 0.1-0.3μ,g/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
NBL1 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.
Target
NBL1
(Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))