PCOLCE Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
PCOLCE Antibody was raised against synthetic peptide QILTNLSKRKCPSQP found near the C Terminus of PCOLCE.
PCOLCE
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 55 kDa band observed in Human Skin and Umbilical Cord lysates (calculated MW of 48.0 kDa according to NP_002584.2). Recommended concentration: 0.3-1μ,g/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
PCOLCE is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.