Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
This antibody is expected to recognize isoform 1 (NP_997700.1) only.
Purification
PRKCB Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
PRKCB Antibody was raised against synthetic peptide DKEFTRQPVELT found at an internal region (near the C Terminus) of PRKCB.
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 80 kDa band observed in lysates of Human Placenta and Rat Brain (calculated MW of 76.9 kDa according to NP_997700.1). Recommended concentration: 0.1-0.3μ,g/mL. Immunohistochemistry: In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration, 1-2μ,g/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
PRKCB is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.