secretogranin II Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
secretogranin II Antibody was raised against synthetic peptide QELGKLTGPNNQKRE found at an internal region of secretogranin II.
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 70 kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 70.9 kDa according to NP_003460.2). Recommended concentration: 0.3-1μ,g/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
secretogranin II is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.