This antibody is expected to recognize both reported isoforms (NP_003273.1, NP_001139313.1). Reported variants represent identical protein: NP_001139301.1, NP_003273.1.
Purification
TNNI2 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
TNNI2 Antibody was raised against synthetic peptide EVRVQKTSKELED found at an internal region of TNNI2.
ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx 25 kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates (calculated MW of 21.3 kDa according to NP_003273.1). Recommended concentration: 1-3μ,g/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
TNNI2 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.