This antibody is expected to recognize both reported isoforms (NP_072097.2, NP_536338.1).
Purification
VPS16 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
VPS16 Antibody was raised against synthetic peptide CYKVQQKDVSDED found at an internal region of VPS16.
VPS16
Reactivity: Human, Mouse, Rat, Cow, Horse, Rabbit, Guinea Pig, Dog, Pig, Monkey, Bat, Hamster
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 95+80 kDa band observed in lysates of cell line HepG2 (calculated MW of 94.7 kDa according to NP_072097.2 and 78.3 kDa according to NP_536338.1). Recommended concentration: 1-3μ,g/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
VPS16 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.
Target
VPS16
(Vacuolar Protein Sorting-Associated Protein 16 Homolog (VPS16))