MAVS antibody (AA 160-450)

Catalog No. ABIN5688365

Product Details anti-MAVS Antibody

Target: MAVS (Mitochondrial Antiviral Signaling Protein (MAVS))

Binding Specificity: AA 160-450

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAVS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Recognizes human Cardif.

Purity: > 95% (SDS-PAGE)

Immunogen: Recombinant human Cardif (aa 160-450).

Clone: Adri-1

Isotype: IgG2b

Application Details

Application Notes: Immunocytochemistry: (1:100). Immunohistochemistry: (paraffin sections). Immunoprecipitation: (1:200). Western Blot: (1:1,000).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for at least 1 year after receipt when stored at -20°C.

Target Details for MAVS

Target: MAVS (Mitochondrial Antiviral Signaling Protein (MAVS))

Alternative Name: Cardif (MAVS Products)

Synonyms: CARDIF antibody, IPS-1 antibody, IPS1 antibody, VISA antibody, D430028G21Rik antibody, Visa antibody, cardif antibody, wu:fj20d04 antibody, zgc:158392 antibody, mitochondrial antiviral signaling protein antibody, MAVS antibody, Mavs antibody, mavs antibody

Background: RIG-I (retinoic acid-inducible gene I, Ddx58) and MDA5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-kappaB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons. Cardif (also called VISA/IPS-1/MAVS) is a new CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and MDA5, leading to the activation of NF-kappaB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-beta production.

Gene ID: 57506

NCBI Accession: NP_065797

UniProt: Q7Z434

Pathways: Activation of Innate immune Response, Inositol Metabolic Process, Hepatitis C