TNFSF13 antibody (AA 93-233)

Catalog No. ABIN5688427

Product Details anti-TNFSF13 Antibody

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Binding Specificity: AA 93-233

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFSF13 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purification: APRIL (monoclonal Aprily-8) antibody was purified to >=95% in SDS-PAGE.

Immunogen: APRIL (monoclonal Aprily-8) antibody was raised against recombinant human APRIL (aa. 93-233) expressed in bacteria.

Clone: Aprily-8

Isotype: IgG1

Application Details

Application Notes: APRIL antibody can be used to recognize human APRIL by Flow Cytometry, immunocytochemistry and Western Blot. Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with 4 % formaldehyde 5 min at RT. After a wash in PBS, samples were dehydrated by washes in 60 %, 80 %, 90 %, 100 % EtOH and xylol. Cells were then dried and embedded in paraffin. Sections were cut, mounted on slides and dried overnight at 50˚,C. Slides were then successively washed 2x 10 min in xylol, 2x 10 min in 100 % EtOH, and then treated 10 min in 100 % MeOH/ 0.6 % H202 to inhibit endogenous peroxidase. Samples were rehydrated by washes in 90 %, 80 %, 60 % EtOH and PBS. After micro-wave treatment, slides were washed 3x in PBS, blocked with IgG, and incubated for 1 hour with 5 μ,g/mL Aprily-8 or control mouse IgG (isotype control) in 1 %BSA / 1x PBS for 1 hour. After PBS washes, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC. Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for human APRIL. Cells (5x105) were incubated on ice for 30 min in 50 μ,l FACS buffer (PBS, 5 % fetal calf serum, 0.02 % azide) containing 10 μ,g/mL of Aprily-8 antibody. After washing in FACS buffer, FITC-conjugated antibody to mouse IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry. Method: 10 μ,g of protein was applied to the gel, and revealed with Aprily-8 (1 μ,g/mL) and HRP-coupled anti-mouse secondary antibody (1:4,000).Note: The hAPRIL construct used in this experiment is an uncleavable fusion protein between hBAFF (aa 1-132) and hAPRIL (aa 93-233). This product is for research use only.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: APRIL antibody can be stored at 4°C, stable for one year.

Target Details for TNFSF13

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Alternative Name: APRIL (TNFSF13 Products)

Synonyms: APRIL antibody, CD256 antibody, TALL-2 antibody, TALL2 antibody, TRDL-1 antibody, ZTNF2 antibody, 2310026N09Rik antibody, April antibody, Tall2 antibody, Trdl1 antibody, SLAN antibody, ANP32B antibody, april antibody, tnf13 antibody, TNLG7B antibody, TNF superfamily member 13 antibody, tumor necrosis factor (ligand) superfamily, member 13 antibody, acidic leucine-rich nuclear phosphoprotein 32 family member B antibody, TNFSF13 antibody, Tnfsf13 antibody, LOC494442 antibody, tnfsf13 antibody

Gene ID: 8741

UniProt: O75888

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response