TNFSF14 antibody (Extracellular Domain)

Catalog No. ABIN5688604

Product Details anti-TNFSF14 Antibody

Target: TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))

Binding Specificity: Extracellular Domain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFSF14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: LIGHT Antibody is supplied as protein A purified IgG2b.

Immunogen: LIGHT antibody was raised against the extracellular domain of human LIGHT.

Clone: 8D4

Isotype: IgG2b

Application Details

Application Notes: LIGHT antibody can be used for detection of CD80 by Western blot at 0.5 - 1 μ,g/mL. LIGHT antibody can be used for immunohistochemistry starting at 2 - 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: LIGHT Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: LIGHT antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for TNFSF14

Target: TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))

Alternative Name: LIGHT (TNFSF14 Products)

Synonyms: TNFSF14 antibody, CD258 antibody, HVEML antibody, LIGHT antibody, LTg antibody, TR2 antibody, HVEM-L antibody, Ly113 antibody, TNF superfamily member 14 antibody, tumor necrosis factor (ligand) superfamily, member 14 antibody, TNFSF14 antibody, Tnfsf14 antibody

Background: LIGHT Antibody: LIGHT, also known as Tumor Necrosis Factor Superfamily member 14 (TNFSF14), is a co-stimulatory molecule that can regulate T-cell activation (1) and has recently been identified as an immune checkpoint protein. LIGHT binds to two different receptors, Herpes Virus Entry Mediator (HVEM) and Lymphotoxin beta Receptor (LTβR). While LIGHT binding to HVEM delivers a co-stimulatory signal to T cells (1), LIGHT binding to LTβR is critical for the formation of lymphoid structures which can stimulate T cell infiltration and activation of a tumor microenvironment, leading to rapid T cell-mediated tissue destruction (2). It has been shown that targeted delivery of LIGHT to tumors, thereby causing the T cell infiltration of the tumor, can enhance the response of the PD-1/PD-L1 checkpoint blockade anti-cancer therapy (3), suggesting that LIGHT may become a potent tool in anti-cancer treatment.

Molecular Weight: Predicted: 26 kDa

Gene ID: 8740

NCBI Accession: NP_003798

UniProt: O43557

Pathways: Cancer Immune Checkpoints