Interferon gamma antibody

Catalog No. ABIN5688726

Product Details anti-Interferon gamma Antibody

Target: Interferon gamma (IFNG)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: This IgG1k antibody was purified on a Protein A affinity column.

Immunogen: The monoclonal antibody was produced in BALB/c x ICR F1 mice using recombinant IFN-gamma as the immunizing antigen.

Clone: 1-1-5A9-2D05

Application Details

Application Notes: ELISA:

In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 4.0-8.0 μ,g/mL of this antibody will detect at least 500 pg/mL of recombinant human IFN-γ, when used with our biotinylated antigen affinity purified anti-human IFN-γ, (XP-5155Bt) as the detection antibody at a concentration of approximately 2.0-4.0 μ,g/mL.

Western Blot:

To detect hIFN-γ, by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIFN-γ, is 1.0-3.0 ng/lane, under non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: 4 °C,-20 °C

Storage Comment: IFN-γ, lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFN-gamma (IFNG Products)

Synonyms: IFG antibody, IFI antibody, IFN-g antibody, Ifg antibody, IFNG2 antibody, IFN-gamma antibody, IFN-G antibody, IFNG antibody, IFNgamma antibody, TCRalpha antibody, INF-G antibody, ifng antibody, interferon gamma antibody, interferon, gamma 1-2 antibody, IFNG antibody, Ifng antibody, ifng1-2 antibody

Gene ID: 3458

UniProt: P01579

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy