G-CSF antibody (Biotin)

Catalog No. ABIN5688999

Product Details anti-G-CSF Antibody (Biotin)

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This G-CSF antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Purification: Anti-hG-CSF specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hG-CSF (human Granulocyte Colony Stimulating Factor).

Application Details

Application Notes: ELISA:

To detect hG-CSF by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hG-CSF.

Sandwich: To detect hG-CSF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human G-CSF (XP-5141) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hG-CSF.

Western Blot:

To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: G-CSF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for G-CSF

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Alternative Name: G-CSF (CSF3 Products)

Synonyms: CSF3 antibody, G-CSF antibody, gcsf antibody, Csfg antibody, MGI-IG antibody, C17orf33 antibody, CSF3OS antibody, GCSF antibody, Gcsf antibody, colony stimulating factor 3 antibody, colony stimulating factor 3 (granulocyte) a antibody, colony stimulating factor 3 (granulocyte) antibody, CSF3 antibody, csf3a antibody, Csf3 antibody

Gene ID: 1991

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization