Oncostatin M antibody (Biotin)

Catalog No. ABIN5689093

Product Details anti-Oncostatin M Antibody (Biotin)

Target: Oncostatin M (OSM)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Oncostatin M antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Purification: Anti-hOncostatin-M specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hOncostatin-M (human Oncostatin-M).

Application Details

Application Notes: ELISA:

To detect hOncostatin-M by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hOncostatin-M.

Sandwich:

To detect hOncostatin M by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human Oncostatin M (XP-5252) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hOncostatin M.

Western Blot:
To detect hOncostatin-M by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hOncostatin-M is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: OncostatinM antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for Oncostatin M

Target: Oncostatin M (OSM)

Alternative Name: OncostatinM (OSM Products)

Synonyms: OSM antibody, OncoM antibody, oncostatin M antibody, OSM antibody, Osm antibody

Gene ID: 5008

UniProt: P13725

Pathways: JAK-STAT Signaling, Negative Regulation of Hormone Secretion