PDGF-BB Homodimer antibody

Catalog No. ABIN5689100

Product Details

Target: PDGF-BB Homodimer

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Purification: Anti-hPDGF-BB specific antibody was purified by affinity chromatography employing immobilized hPDGF-BB matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPDGF-BB.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-BB (10.0 ng/mL), a concentration of 0.05 - 0.07 μ,g/mL of this antibody is required.

ELISA:

To detect hPDGF-BB by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hPDGF-BB.

Sandwich:

To detect hPDGF-BB by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human PDGF-BB (XP-5255Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-BB.

Western Blot:

To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: PDGF-BB antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details

Target: PDGF-BB Homodimer

Alternative Name: PDGF-BB

Synonyms: PDGF-2 antibody, PDGF2 antibody, SIS antibody, SSV antibody, c-sis antibody, PDGF-B antibody, Sis antibody, platelet derived growth factor subunit B antibody, platelet derived growth factor, B polypeptide antibody, PDGFB antibody, Pdgfb antibody

Gene ID: 5155

UniProt: P01127