CXCL12 antibody
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- Target See all CXCL12 Antibodies
- CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CXCL12 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Neutralization (Neut)
- Purification
- Anti-hSDF-1-alpha specific antibody was purified by affinity chromatography employing immobilized hSDF-1-alpha matrix.
- Immunogen
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hSDF-1-alpha (human Stromal Cell-Derived Factor-1-alpha).
- Top Product
- Discover our top product CXCL12 Primary Antibody
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- Application Notes
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hSDF1α, (100 ng/mL), a concentration of 2-4 μ,g/mL of this antibody is required.
ELISA:
To detect hSDF-1-alpha by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hSDF-1-alpha.
Sandwich:
To detect hSDF-1α, by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human SDF-1α, (XP-5269Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSDF-1α,.
Western Blot:
To detect hSDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSDF-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Storage
- -20 °C
- Storage Comment
- SDF-1a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- Target
- CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))
- Alternative Name
- SDF-1a (CXCL12 Products)
- Synonyms
- IRH antibody, PBSF antibody, SCYB12 antibody, SDF1 antibody, TLSF antibody, TPAR1 antibody, CINC-1 antibody, Gro1 antibody, Pbsf antibody, Scyb12 antibody, Sdf1 antibody, Tlsf antibody, Tpar1 antibody, Fsp antibody, KC antibody, Mgsa antibody, N51 antibody, Scyb1 antibody, gro antibody, sdf-1 antibody, sdf1 antibody, xSDF-1 antibody, SDF-1 antibody, cxcl12 antibody, sdf-1a antibody, sdf1a antibody, wu:fa55e10 antibody, wu:fc16h12 antibody, wu:fj84c02 antibody, C-X-C motif chemokine ligand 12 antibody, C-X-C motif chemokine ligand 1 antibody, chemokine (C-X-C motif) ligand 12 antibody, chemokine (C-X-C motif) ligand 1 antibody, C-X-C motif chemokine ligand 12 L homeolog antibody, chemokine (C-X-C motif) ligand 12a (stromal cell-derived factor 1) antibody, CXCL12 antibody, cxcl12 antibody, Cxcl1 antibody, Cxcl12 antibody, cxcl12.L antibody, cxcl12a antibody
- Gene ID
- 6387
- UniProt
- P48061
- Pathways
- Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling
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