VEGF antibody

Catalog No. ABIN5689125

Product Details anti-VEGF Antibody

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEGF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Anti-hVEGF specific antibody was purified by affinity chromatography employing immobilized hVEGF matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hVEGF (human Vascular Endothelial Cell Growth Factor).

Application Details

Application Notes:

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hVEGF (10 ng/mL), a concentration of 0.05 - 0.1 μ,g/mL of this antibody is required.

ELISA:

To detect hVEGF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hVEGF.

Sandwich:

To detect hVEGF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human VEGF (XP-5291Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVEGF.

Western Blot:
To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: VEGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for VEGF

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Alternative Name: VEGF (VEGF Products)

Synonyms: MVCD1 antibody, VEGF antibody, VPF antibody, Vegf antibody, Vegf120 antibody, Vegf164 antibody, Vegf188 antibody, Vpf antibody, VEGF-A antibody, VEGF164 antibody, eVEGF120 antibody, eVEGF164 antibody, vegf-a antibody, vegf antibody, vascular endothelial growth factor A antibody, vascular endothelial growth factor A L homeolog antibody, vascular endothelial growth factor antibody, vascular endothelial growth factor precursor antibody, VEGFA antibody, Vegfa antibody, vegfa.L antibody, VEGF antibody, vegf antibody

Gene ID: 7422