PK (pThr446) antibody
Reactivity: Human
IF, IHC, WB
Host: Rabbit
Polyclonal
unconjugated
-
- Target
- PK
-
Binding Specificity
- pThr446
- Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- Un-conjugated
- Application
- Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- This antibody detects endogenous level of PKR only when phosphorylated at threonine 446.
- Purification
- Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
- Immunogen
- PKR (Phospho-Thr446) antibody was raised against a peptide sequence around phosphorylation site of threonine 446 (K-R-T (p) -R-S) derived from Human PKR.
-
-
- Application Notes
- Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store antibody at -20°C for up to one year.
-
- Target
- PK
- Background
- Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
- Molecular Weight
- 68 kDa
- Gene ID
- 5610
- NCBI Accession
- NP_001129123
- UniProt
- P19525
-
You are here:
