DOK2 antibody (pTyr299)

Catalog No. ABIN5689839

Product Details anti-DOK2 Antibody

Target: DOK2

Binding Specificity: pTyr299

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificity: This antibody detects endogenous level of p56Dok-2 only when phosphorylated at tyrosine 299.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Immunogen: p56Dok-2 (Phospho-Tyr299) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 299 (G-E-Y (p) -A-V) derived from Human p56Dok-2.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for DOK2

Target: DOK2

Alternative Name: p56Dok 2 (DOK2 Products)

Synonyms: DokR antibody, Frip antibody, Dok-2 antibody, p56DOK antibody, p56dok-2 antibody, docking protein 2 antibody, DOK2 antibody, Dok2 antibody

Background: DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation

Molecular Weight: 56 kDa

Gene ID: 9046

NCBI Accession: NP_003965

UniProt: O60496