APOBEC3B antibody (AA 20-70)

Catalog No. ABIN5689877

Product Details anti-APOBEC3B Antibody

Target: APOBEC3B (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3B (APOBEC3B))

Binding Specificity: AA 20-70

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOBEC3B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: APOBEC3B antibody is human, mouse and rat reactive . At least three isoforms of APOBEC3B are known to exist, this antibody will detect all three. APOBEC3B antibody may cross-react with APOBEC3D.

Purification: APOBEC3B antibody is affinity chromatography purified via peptide column.

Immunogen: APOBEC3B antibody was raised against a 14 amino acid peptide near the amino terminus of human APOBEC3B.

The immunogen is located within amino acids 20 - 70 of APOBEC3B.

Isotype: IgG

Application Details

Application Notes: APOBEC3B antibody can be used for detection of APOBEC3B by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: APOBEC3B Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: APOBEC3B antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for APOBEC3B

Target: APOBEC3B (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3B (APOBEC3B))

Alternative Name: APOBEC3B (APOBEC3B Products)

Synonyms: A3B antibody, APOBEC1L antibody, ARCD3 antibody, ARP4 antibody, DJ742C19.2 antibody, PHRBNL antibody, bK150C2.2 antibody, APOBEC3B antibody, Apobec3 antibody, Apobec3f antibody, APOBEC3F antibody, APOBEC3Z2 antibody, APOBEC3Z3 antibody, apolipoprotein B mRNA editing enzyme catalytic subunit 3B antibody, APOBEC3B antibody, Apobec3b antibody

Background: The Apolipoprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytosine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates (1). Similar to TRIM5? which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection (2,3). APOBEC3B has also been shown to be upregulated in several cancers, it is thought that APOBEC3B-catalyzed genomic uracil lesions are responsible for a large proportion of mutations in multiple distinct cancers (4).

Molecular Weight: Predicted: 42 kDa

Observed: 48 kDa

Gene ID: 9582

NCBI Accession: NP_004891

UniProt: Q9UH17