APOBEC3B antibody is human, mouse and rat reactive . At least three isoforms of APOBEC3B are known to exist, this antibody will detect all three. APOBEC3B antibody may cross-react with APOBEC3D.
Purification
APOBEC3B antibody is affinity chromatography purified via peptide column.
Immunogen
APOBEC3B antibody was raised against a 14 amino acid peptide near the amino terminus of human APOBEC3B. The immunogen is located within amino acids 20 - 70 of APOBEC3B.
APOBEC3B antibody can be used for detection of APOBEC3B by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
APOBEC3B Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
APOBEC3B antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Target
APOBEC3B
(Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3B (APOBEC3B))
The Apolipoprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytosine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates (1). Similar to TRIM5? which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection (2,3). APOBEC3B has also been shown to be upregulated in several cancers, it is thought that APOBEC3B-catalyzed genomic uracil lesions are responsible for a large proportion of mutations in multiple distinct cancers (4).