CTGF antibody

Catalog No. ABIN5689933

Product Details anti-CTGF Antibody

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTGF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Purification: CTGF antibody was purified by affinity chromatography employing immobilized CTGF matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hCTGF. Human CTGF specific antibody was purified by affinity chromatography employing immobilized hCTGF matrix.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hCTGF (5.0 μ,g/mL), a concentration of 6.0 - 9.0 μ,g/mL of this antibody is required.

ELISA:
Indirect:
To detect hCTGF by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCTGF.
Sandwich:
To detect hCTGF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required.

Western Blot:
To detect hCTGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: CTGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CTGF

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Alternative Name: CTGF (CTGF Products)

Synonyms: Ccn2 antibody, Fisp12 antibody, Hcs24 antibody, fisp-12 antibody, CCN2 antibody, HCS24 antibody, IGFBP8 antibody, NOV2 antibody, CTGRP antibody, ctgf antibody, hm:zeh1559 antibody, si:dkey-266k12.4 antibody, wu:fl25c03 antibody, zeh1559 antibody, zgc:136644 antibody, XCTGF antibody, ccn2 antibody, nov2 antibody, hcs24 antibody, igfbp8 antibody, CTGF antibody, si:ch211-173b8.2 antibody, connective tissue growth factor antibody, connective tissue growth factor a antibody, connective tissue growth factor L homeolog antibody, connective tissue growth factor b antibody, Ctgf antibody, CTGF antibody, ctgfa antibody, ctgf.L antibody, ctgf antibody, ctgfb antibody

Background: CTGF (connective tissue growth factor) is a 38  kDa, cysteine-rich, secreted peptide. It is a new member of the peptide family that include serum-induced immediate early gene products, a v-src-induced peptide and a putative proto-oncogene. Among the many functions of the CTGF gene family are embryogenesis, wound healing and regulation of extracellular matrix production.

Gene ID: 1490

UniProt: P29279

Pathways: Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Growth Factor Binding